The key aroma compound of scented rice identified as 2-acetyl-1-pyrroline (2AP) has been reported by many researchers that it was present in rice grain and some processed food products at a very low concentration level, 0.01-3.0 ppm. As a key index for evaluation of aroma quality of rice, a fast and accurate quantitative method for determination of 2AP in a large numbers of rice samples is essential. In this research, methods for quantification of 2AP in rice samples including grain, leaf and spikelet were investigated and developed. These developed methods which have not been reported for their uses in 2AP quantitative approach divided into four analytical procedures. Three of them employed currently available techniques for extraction and subsequent chromatographic analysis utilizing analytical instruments. Whereas in the last simplified method, colorimetric technique was employed in order to minimize instrument cost. The first quantitative method for 2AP in rice samples developed in this study comprised solvent extraction using acidic solution followed by re-extraction into organic solvent, dichloromethane, before subjected to analysis by gas chromatography (GC) with flamed ionization detection (FID). It has advantages over the conventional method which emphasized on the use of steam distillation for extraction of the rice volatiles prior to GC analysis that heat associated in extraction step was eliminated, less complexity of the extract obtained, less amount of rice sample required as well as less analysis time. In the second and third methods, two techniques of gas extraction, so called headspace sampling, for extraction of the rice volatiles were employed; static headspace sampling automated with GC-FID and solid phase microextraction (SPME) prior to GC-FID. The automated static headspace GC-FID was proved as rapid and suitable for routine analysis. These methods reduced sample extraction and preparation steps and the chemicals as used in the wet extraction were excluded thus considered environmental friendly. The fourth method for quantitative analysis of 2AP utilized a specific chemical reaction between 2AP and a reagent producing a color product. Amount of 2AP in the sample was determined by measurement of the color intensity using a UV-Visible spectrophotometry. Each developed method was validated and their applications to quantification of 2AP in some aromatic and non-aromatic rice samples were performed. Analytical efficiency and applicability of these developed methods were comparatively evaluated.