Tuberculosis ({TB)} is a communicable disease caused by the bacterium Mycobacterium tuberculosis ({MTB)} and is a persistent problem in the developing countries. Loop-mediated isothermal amplification ({LAMP)} allows {DNA} to be amplified rapidly at a constant temperature. Here, a {LAMP} method was combined with a chromatographic lateral-flow dipstick ({LFD)} to detect {IS6110} gene of M. tuberculosis specifically and rapidly. The reaction was optimized at {63°C} for 60 min, and the amplified {DNA} hybridized to an {FITC-labeled} oligonucleotide probe for 5 min was detected at the {LFD} test line 5 min after application. Excluding the step of {DNA} extraction, the test results could be generated approximately within 1 h. In addition to the advantage of short assay time, this technique could avoid the contact of carcinogenic ethidium bromide due to the exclusion of the electrophoresis analysis step. Furthermore, the data indicated that {LAMP-LFD} could detect M. tuberculosis genomic {DNA} as little as 5 pg. The technique showed a significant specificity since no cross-hybridization to M. intracellulare ({MIC)}, M. fortuitum ({MFT)}, M. avium ({MAV)}, M. kansasii ({MKS)}, and M. gordonae ({MGD)} genomic {DNAs} was observed. In the clinical unknown samples test, the sensitivity of {LAMP-LFD} was 98.92 % and the specificity was 100 % compared to those of the standard culture assay. Based on its sensitivity, specificity, rapidity, low cost, and convenience, {LAMP-LFD} could be applicable for use in both laboratories and epidemiological surveys of {MTB.}