Soil is one of the resources containing a variety of microorganisms. It was estimated that only 1% of microorganisms could be identified by culturing method whereas 99% of microorganisms could not be cultivated. Therefore, any novel proteins, especially valuable enzymes, produced from the latter group of microorganisms are yet to be found. Starch-degrading enzymes are groups of enzymes that have been widely used for starch processing industries. A number of these enzymes have been characterized from a variety of microorganisms including soil bacteria. As many processes in the industries have to be completed at high temperature, the identification of thermostable enzymes is increasingly focused. This type of enzymes can be isolated from thermophillic archaebacteria, which could be found in extreme environments. Therefore, this work aimed to identify genes encoding starch-degrading enzymes from the particular environment, especially, at high temperature. Hot springs in Thailand are one of the local areas that could contain variety of microorganisms that produce such valuable enzymes. In this work, the genomic DNA will be extracted from the soils collected from hot springs and purified. The part of starch-degrading enzyme genes will be obtained by PCR-amplified using degenerate primers. The PCR products will be subcloned in a vector plasmid and transferred into Escherichia coli. The DNA inserts will be sequenced in order to identify the ones that harbor partial genes of starch-degrading enzymes. The DNA fragments correspond to the enzyme genes could then be used as probes to obtain whole sequences of these enzymes.