Objective: To investigate the effect of intraperitoneal injection of various doses of plant leaves extract at 48 hours and 7 days; and to investigate the effect of dietary supplement of various doses of dry plant leaves at 1 and 2 months. Method: Fish in each group was evaluated in term growth rate; macrophage studies; nitroblue tetra zolium test; lysozyme activity test; micronucleus and nuclear abnormality tests; hematology studies; liver function test; histopathological and ultrastructural studies. Result: The results had shown the time-dose dependent, the higher dose the more effect and the longer time the higher response. The administration route via the food supplement was the greater than the intraperitonial injection. The highest efficiency of leaf extract was T. laurifolia follow by P. granatum and P. guajava. Conclusion: In conclusion, the results presented in this study show no harmful of these leaf extracts.