The aqueous extract of Cryptolepis buchanani leaves was tested against food-borne pathogen bacteria (S. aureus ATCC 25923, E. coli ATCC 25922 and S. typhimurium ATCC 14028), nosocomial infection bacteria (K. pneumoniae ATCC 10031, P. vulgaris ATCC 13315 and(S. aeruginosa ATCC 9721) and normal flora bacteria (L. plantarum ATCC 14917 and S. epidermidis ATCC 12228). The plant aqueous extract showed inhibitory effect against S. aureus ATCC 25923, E. coli ATCC 25922, S. typhimurium ATCC 14028, K. pneumoniae ATCC 10031, P. vulgaris ATCC 13315, B. subtilis ATCC 6633, L. plantarum ATCC 14917 and S. epidermidis ATCC 12228. The MICs (Minimal Inhibitory Concentrations) and MBCs (Minimal Bactericidal Concentrations) of this plant against all tested bacteria are in the range of 1-16 and 2-32 g L^-1, respectively. In conclusion, C. buchanani leaves aqueous extract showed broad-spectrum antimicrobial activity against food-borne pathogen bacteria, nosocomial infection bacteria and some normal flora bacteria at low concentration. This may supported the used of C. buchanani aqueous extract as food-borne pathogen bacterial growth control additive and nosocomial infections treatment remedy. © 2009 Academic Journals Inc.