The carbon estimates in this dataset were taken from “Wololla” a cattle property in the Desert Uplands region. The property is located approximately 20 kilometres south-west of Jericho and is located within the Desert Uplands Bioregion, and includes vegetation categorised in 17 Regional Ecosystems. As the property included such a wide range of Regional Ecosystems (REs) it was decided to sample only three of the REs, and to apply the information from these and other sites on similar properties, to the other REs. At each RE one general area (site) was selected to be representative of the vegetation. Trees were measured in 200m2 rectangular plots called transects. 30 transects were laid out at each site. Each transect was 50 metres long and 4 metres wide, and all were laid in a north-south direction. All trees over 1.8 m were measured in the first three transects. The stem circumference of each tree selected was measured at a height of 30 cm above the ground. From this measurement, the tree biomass was calculated using previously developed equations, which relate stem circumference, or in some cases, stem diameter, 7 to total above-ground biomass. The dataset contains all above and below ground tree carbon data for the property and associated calculations.