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High-throughput polymerase chain reaction in parallel circular loops using magnetic actuation

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : High-throughput polymerase chain reaction in parallel circular loops using magnetic actuation
นักวิจัย : Sun, Yi , Nguyen, Nam-Trung , Kwok, Yien Chian
คำค้น : DRNTU::Engineering::Mechanical engineering.
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2551
อ้างอิง : Sun, Y., Nguyen, N. T. & Kwok, Y. C. (2008). High-Throughput Polymerase Chain Reaction in Parallel CircularLoops Using Magnetic Actuation. Analytical Chemistry, 80(15), 6127-6130. , http://hdl.handle.net/10220/7841 , http://dx.doi.org/10.1021/ac800787g , 134352
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Analytical chemistry
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

We report here a novel multichannel closed-loop magnetically actuated microchip for high-throughput polymerase chain reaction (PCR). This is achieved by designing a series of concentric circular channels on one microchip and exploiting a magnetic force to drive DNA samples flowing continuously through the closed loops. The magnetic force arises from an external permanent magnet through ferrofluid plugs inside the microchannels. The magnet enables simultaneous actuation of DNA samples in all the channels. As the samples go around the loops, they pass through three preset temperature zones. Parameters of PCR, such as incubation time, temperatures, and number of cycles, can be fully controlled and adjusted. High reproducibility was achieved for different channels in the same run and for the same channels in consecutive runs. Genetically modified organisms (GMOs) were amplified simultaneously using the developed device. This simple, reliable, and high-throughput PCR microchip would find wide applications in forensic, clinical, and biological fields.

บรรณานุกรม :
Sun, Yi , Nguyen, Nam-Trung , Kwok, Yien Chian . (2551). High-throughput polymerase chain reaction in parallel circular loops using magnetic actuation.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Sun, Yi , Nguyen, Nam-Trung , Kwok, Yien Chian . 2551. "High-throughput polymerase chain reaction in parallel circular loops using magnetic actuation".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Sun, Yi , Nguyen, Nam-Trung , Kwok, Yien Chian . "High-throughput polymerase chain reaction in parallel circular loops using magnetic actuation."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2551. Print.
Sun, Yi , Nguyen, Nam-Trung , Kwok, Yien Chian . High-throughput polymerase chain reaction in parallel circular loops using magnetic actuation. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2551.