Embryo cryopreservation can assist banking of transgenic (TG) thalassemic mouse lines. The purpose of this study was to evaluate pups rate, weanling weight, post-weanling growth rate,and hematological parameters of the four TG pups (BKO, 654, E2, and E4) obtained from vitrifiedwarmed embryos transfer. Two and eight-cell embryos were produced by breeding four lines of TG thalassemic males to wild-type (WT) females (C57BL/6J) and were cryopreserved by vitrification in straws using 35% ethylene glycol. Percentage of pups developed from cryopreserved 2-cell embryos in 654, BKO, E2, and E4 strains were 32.5, 31.5, 20.0, and 18.1%, respectively. Since there are many dead offsprings which are often eaten in 8-cell embryos experiment, in vivo development of fresh and vitrified embryos after embryo transfer can be observed from implantation and fetus rates only. The result showed that vitrification was not influent to the implantation rates of 654, BKO and E2, while E4 had a significant reduction in implantation rate. The weanling weight (3 weeks (wk) of age), post-weanling growth rate (4 to 8 wk of age), hematological parameters, and spleen index were measured only in TG and WT viable pups from cryopreserved 2-cell embryos. Significant differences in weanling weight were found between WT and TG pups of BKO and 654 lines. At post-weaning BKO and 654 lines, TG mice were significantly lighter than their agematched WT mice. However, significant differences in weanling and post-weanling weight were not found between WT and TG pups of E2 and E4 lines. The hematological parameters values showed anemia, thalassemia phenotype, in BKO and 654 mice, but not in E2 and E4 mice. Moreover, the TG mice of BKO and 654 showed higher spleen index than WT, E2 and E4 mice. In conclusion, thalassemic mouse lines affected pups rate and thalassemia phenotypes, such as anemia, high spleen index, low weanling and post-weanling weight were evident only the two TG genetic background in BKO and 654.