Bacillary dysentery (shigellosis) caused by Shigella species is a public health problemin many developing countries. Food and drink contaminated with the bacteria areoften the source of the shigellosis epidemic's spread across communities. In this studybacteriophage specific to Shigella dysenteriae DMS2137 was collected from sewagewater in Ubon Ratchathani province, Thailand. The bacteriophage was purified andcharacterized. It was able to infect only S. dysenteriae strain DMS2137. The phagewas stable at temperatures 70 °C for 3 h and at pH 4-11 overnight. In addition it wasstable in TSB for over 2 months at room temperature. The morphology ofbacteriophage under transmission electron microscopy (TEM) consisted of anicosahedral head (diameter 65–70 nm), and rigid long tail (20–22 nm × 67–70 nm).This bacteriophage may be useful for biological control, therapeutic or diagnosticagent of S. dysenteriae in animals,environmental water reservoirs and foodproduction.