Uterine samples were collected from Addis Ababa abattoir with the objective of isolating aerobic bacteria and determine the antibiotic sensitivity profiles of the isolates. A total of 38 uteri of cows were collected and samples were processed for bacteriology and isolates identified by morphological, staining and biochemical tests. At least one species of bacteria was isolated from each uterine sample. The highest isolate found in this study was Escherichia coli (42.1%) followed by Bacillus species (17.1%), Staphylococcus spp. (15.7%), Arcanobacterium pyogens (7.9%) and Streptococcus spp. and Proteus spp. (3.9% each). Other isolates include Corynebacterium, Rhodococcusequi, Klebsiela and Citrobacter. Among all bacteria screened, sensitivities for selected drug were: chloramphenicol (98.6%), amikacin (90.8%), nitrofurantoin (82.9%) and trimethoprim-sulfamethoxasole (81.5%), tetracycline (71%) and polymixin-B (67.1%). Contrary to this, penicillin-G, methicillin and erythromycin showed highest resistance to the isolates identified respectively.