This article aims to look into a successful Triple-Helix based project in Thailand, namely “Khonkaen One Stop Services”. The project is under the coordination between Thai Software Industry Promotion Agency (SIPA), Faculty of Informatics, Mahasarakham University, Khonkaen provincial authority and several small and medium software companies. The initiative has started from the mission of SIPA to promote software industry in the northeastern of Thailand together with the mission of the Khonkaen provincial authority to extend the Information Communication Technology (ICT) usage to enhance citizen services and provincial administration tasks. To accomplish the objectives, Khonkaen province and SIPA have agreed to employ an ICT expert team from Faculty of Informatics Mahasarakham University to analyze and develop the core system. The know-how is then transferred to the regional software companies to learn and get extensive jobs from the local government sector. By applying the Triple Helix model of university-industry-government relations on the project, University, industry, government can coordinate successfully and fulfill their missions.