Crude extracts of mushroom Phellinus linteus were investigated for their antioxidant, cytotoxic, antimalarial and antibacterial activities. The crude EtOAc fraction from large scale extraction showed the strongest antioxidant activity with IC₅₀ value of 17.73±0.27 μg mL^-1 and the highest total phenolic value of 76.39±0.07 EGA. This activity was as good as (p>0.05) L(+)ascorbic acid (IC₅₀ value of 16.56±0.50 μg mL^-1). From small scale extraction, IC ₅₀ values of crude extracts were in the range of 24.15±0.50 to 207.02±1.95 μg mL^-1 and the values of total phenolic content ranged from 68.11±0.06 to 5.96±0.18 EGA. Crude MeOH, CH₂Cl₂ and EtOAc extracts from large scale extraction exhibited cytotoxicity against MFC7 and NCI-H187 cancer cells. Crude MeOH and CH₂Cl₂ extracts also displayed antimalarial activity against Plasmodium falciparum with IC₅₀ values of 3.15 and 3.08 μg mL^-1, respectively. None of the extracts were found to have antibacterial activity against five different species of pathogenic bacteria. © 2009 Asian Network for Scientific Information.