This study aimed to prepare Silk Fibroin (SF) films with different weight (0.5, 1 and 1.5%) for loading chlorhexidine diacetate as substrate for study their characteristics including morphology, structure and thermal properties. The morphological observation under scanning electron spectroscopy found that all of films have a smooth surface. With cross-section micrographs, the lowest SF content was smoother texture than other. The secondary structures of various films were determined by Fourier transform infrared (FTIR) spectrometer. The results showed that SF film composed of β-sheet structures in different ratio depending on the SF content. The result suggested that high SF content formed crystalline by interaction between amino acids molecules in higher ratio than other. However, thermal properties of the SF films did not dramatically differ compared between low and high SF content. It was also found that CHX did not affect on the SF characteristics. © 2009 Asian Network for Scientific Information.