Blood-feeding and autogenous sub-colonies were selected from a laboratory, stock colony of Aedes togoi, which was originally collected from Koh Nom Sao, Chanthaburi province, Southeast Thailand. Comparative biology and filarial susceptibility between the two sub-colonies (blood-feeding: F₁₁, F₁₃; autogeny: F₃₈, F₄₀) were investigated to evaluate their viability and vectorial capacity. The results of comparison on biology revealed intraspecific differences, i.e., the average egg deposition/gravid female (F₁₁/F₃₈; F₁₃/F₄₀), embryonation rate (F₁₃/F₄₀), hatchability rate (F₁₁/F₃₈; F₁₃/F₄₀), egg width (F₁₁/F₃₈), wing length of females (F₁₃/F₄₀), and wing length and width of males (F₁₁/F₃₈) in the blood-feeding sub-colony were significantly greater than that in the autogenous sub-colony; and egg length (F₁₁/F₃₈) and width (F₁₃/F₄₀), and mean longevity of adult females (F₁₁/F₃₈) and males (F₁₃/F₄₀) in the blood-feeding sub-colony were significantly less than that in the autogenous sub-colony. The results of comparison on filarial susceptibility demonstrated that both sub-colonies yielded similar susceptibilities to Brugia malayi [blood-feeding/autogeny = 56.7% (F₁₁)/53.3%(F₃₈), 60%(F₁₃)/83.3%(F₄₀)] and Dirofilaria immitis [blood-feeding/autogeny = 85.7%(F₁₁)/75%(F₃₈), 45%(F₁₃)/29.4%(F₄₀)], suggesting autogenous Ae. togoi sub-colony was an efficient laboratory vector in study of filariasis.