Aim: To investigate the antioxidant activity and total phenolic content from the crude extracts and crude fractions of ten species of Phellinus mushrooms collected from northeast Thailand. Methods: The samples were tested for their radical scavenging activity toward 2, 2-diphenyl-1-pricylhydrazyl (DPPH) radical (DPPH method) and total phenolic content (Folin-Ciocalteu method). Results: Some of the investigated extracts exhibited potent radical scavenging activity with the IC ₅₀ ranging from (7.30 ± 0.34) to (19.80 ± 0.13) μg·mL ^-1. IC ₅₀ were in the range of the standard antioxidant used; quercetin, ascorbic acid and butylated hydroxytoluene (BHT). The strongest scavenging activity as comparable to quercetin was found in the crude 80% EtOH extract of P. torulosus. The crude EtOAc fraction of P. pini showed the highest total phenolic content with a value of 87.76 ± 1.00 equivalent gallic acid (EGA) while the samples with potent antioxidant activity were also determined to have high amount of total phenolics (78.34 ± 0.27 to 51.01 ± 0.38) EGA. Conclusion: These results indicated that crude extracts from Phellinus mushrooms have a potential to be natural antioxidant source. © 2011 China Pharmaceutical University.