Silk fibroins (SF) were prepared from various kinds of domestic Thai silk, locally called Nanglai, Kaki and Mo varieties. They were then dissolved into aqueous solutions and used as substrate for blending with gelatin (G). The blend films were performed by mixing SF and G solutions at 1:1 ratio before pouring on plastic plates. The plates were dried at 40°C in an oven for 3 days. Morphology, secondary structures and thermal properties of the blend films were then investigated by using scanning electron microscope (SEM), Fourier transform infrared (FTIR) spectroscopy and thermogravimetric analyzer (TA), respectively. The texture of blend films was homogeneously appeared without phase separation. All blend film slightly rough of their surfaces than the native films. FTIR results indicated that interactions via hydrogen bonds between SF and G were found. All of blend film composed of both α-helix mixed β-sheet structures. Each SF/G blend films have variation of their thermal properties. G blend film with SF from Mo variety has the lowest of maximum temperature of decomposition while Nanglai was the highest. The SF/G blend films loaded methylene blue (MB) was also performed for in vitro releasing and dissolution study. The results found that MB was rapidly released from the blend film of SF from Mo variety and G compared to other films. However, this film was lower dissolution at the initial time and then converted to higher dissolution until the final of study. The results suggested that G blend with Thai SF should be applied for drug controlled release by adjusting the variety of silk as well as drug characteristics. © Research India Publications.