This study investigated the antioxidant and anti-inflammatory activities of -oryzanol rich extracts from Thai glutinous purple rice bran. Five purple rice bran samples, PAH E-KAW, GAM PEUAK, NIAW DAM, GAM THOR and GAM BOUNG were obtained from local glutinous cultivars in Northern Thailand. The -oryzanol rich extracts were prepared by soxhlet extraction and semi-purified by column chromatography. The extracts contained -oryzanol in the range of 1.23 to 9.14% w/w. The extracts containing more than 5% w/w of -oryzanol (PAH E-KAW, GAM THOR and GAM BOUNG) were selected for investigation of antioxidant and anti-inflammatory activities; and first standardized by adding the equivalent amount of each extract to give 10% w/v -oryzanol. All of extracts exerted moderate ABTS + and superoxide anion scavenging activity. GAM BOUNG exhibited the highest ABTS + scavenging activity with VCEAC value of 201.3  1.84 mg L-ascorbic acid/ gram extract, TEAC value of 267.2  2.96 mg Trolox/ gram extract and QEAC value of 137.4  3.41 mg Quercetin/ gram extract followed by PAH E-KAW and GAM THOR, respectively. GAM BOUNG also showed the highest activity on superoxide anion scavenging at IC 50 = 12.56  0.13 g/ml followed by GAM THOR and PAH E-KAW. Furthermore, GAM BOUNG exhibited a strong inhibition effect on linoleic acid peroxidation (IC 50 = 36.79  2.97 g/ml). Additionally, all of the extracts showed a strong anti-inflammatory activity through inhibitory effect on nitric oxide (NO) production in combined LPS-IFN--activated RAW 264.7 murine macrophage cells. GAM BOUNG exhibited the highest inhibitory effects on NO production (IC 50 = 29.32  2.21 g/ml), without exerting cytotoxicity followed by GAM THOR and PAH E-KAW. These results indicate that -oryzanol rich extracts from Thai glutinous purple rice bran are acting as a lipophilic radical scavenger better than a hydrophilic radical scavenger. Moreover, these extracts exert a potent anti-inflammatory activity.