Thesis (Ph.D.)--Chulalongkorn University, 2011

Doxycycline and monosodium glutamate (MSG) loaded CHI/PLA/PCL blendfilm was prepared by the modified spontaneous emulsification-solvent diffusion method. The results in this study clearly revealed that the blend film prepared could be possibly used as a device to deliver the active ingredients to the targeted human organ which in this case was the skin with an opened wound. From the film characteristic test, it was found that the CHI/PLA/PCL blend film containing 60% CHI, 28% PLA, and 12% PCL exhibited the good film properties for making the dressing device. Doxycycline/MSG loaded CHI/PLA/PCL blend film could rapidly deliver both doxycycline and MSG at the high release percentage approaching 100% of the amounts loaded. It was observed that MSG accelerated blood clotting and fibrin formation; thus, it demonstrated the good hemostatic activity. The antibacterial activity of doxycycline loaded CHI/PLA/PCL blend film against the gram positive Staphylococcus aureus and the gram negative Escherichia coli was also investigated. It was cleared that doxycycline released from the blend film played the crucial role in bacterial inhibition as observed from the lowest bacterial cell dry weight observed when compared with the control bacterial culture or the bacterial cultures with the presence of other film studied.