Thesis (M.Sc.)--Chulalongkorn University, 2013

Purpose: The objective of this study was to investigate the allele frequencies and genotype distributions of +45T/G (rs2241766) and +276 G/T (rs1501299) polymorphisms in AdipoQ gene, plasma adiponectin levels, and their associations in Thai knee osteoarthritis (OA) patients. Methods: Plasma samples were collected from 105 patients and 94 controls. Genomic DNA was isolated from peripheral blood of 202 patients and 196 controls. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect genotypes of +45T/G and +276G/T. The enzyme-linked immunosorbent assay (ELISA) was used to measure plasma adiponectin levels. Results: No statistically significant differences were identified between the two groups with respect to genotype distributions and allele frequencies of +45T/G and +276G/T (P>0.05). For +276G/T, significant differences were identified between Kellgren-Lawrence (KL) grade 2 and grade 3 (P=0.046), and between KL grade 2 and grade 4 (P=0.037) patients in the BMI