AIM: Comparisons between reactive agility tests incorporating generic and sport-­specific stimuli have been performed only in field-­based team sports. The aim of this study was to compare generic (light-­based) and sport-­specific (live opponent) reactive agility tests in court-­based team sport athletes. METHODS: Twelve semi-­professional male basketball players (age: 25.9 ± 6.7 yr; stature: 188.9 ± 7.9 cm; body mass: 97.4 ± 16.1 kg; predicted maximal oxygen uptake: 49.5 ± 5.3 mL∙kg-­1∙min-­1) completed multiple trials of a Reactive Agility Test containing light-­based (RAT-­Light) and opponent-­based stimuli (RAT-­Opponent). Multiple outcome measures were collected during the RAT-­Light (agility time and total time) and RAT-­Opponent (decision time and total time). RESULTS: Mean performance times during the RAT-­Light (2.233 ± 0.224 s) were significantly (P < 0.001) slower than during the RAT-­Opponent (1.726 ± 0.178 s). Further, a small relationship was observed between RAT-­Light agility time and RAT-­Opponent decision time (r10 = 0.20), while a trivial relationship was apparent between total performance times across tests (r10 = 0.02). Low commonality was observed between comparable measures across tests (R2 = 0-­4%). CONCLUSION: Reactive agility tests containing light-­based and live opponent stimuli appear to measure different qualities in court-­based team sport athletes. Court-­based team sport coaches and conditioning professionals should not use generic and sport-­specific reactive agility tests interchangeably during athlete assessments.