The flowers of Chrysanthemum morifolium afforded mixtures of the C-3 palmitate and myristate esters (3:2) of mailadiol 1, the C-3 palmitate and myristate esters (3:2) of heliantriol C2, and fatty acid esters (1:1) of faradiol 3 and arnidol 4. The structures of 1 and 2 were elucidated by extensive 1D and 2D NMR analyses and MS while 3 and 4 were identified by comparison of their H NMR spectral data with those found in the literature. Traces of the stearate esters of 1 and 2 were also detected by MS. Antimicrobial tests indicated that 1 has moderate activity against Aspergillus niger and Pseudo aeruginosa, low activity against Trichophyton mentagrophytes, Candida albicans, and Bacillus subtillis, and inactive against Staphylococcus aureus and Escherichia coli. Compound 2 has moderate activity against A. niger, and low activity against P. aeruginosa and S. aureus, and inactive against B. subtillis and E. coli.