We investigated the role of GlyBet (100 mM) applied exogenously in alleviating water-deficit induced stress in indica rice cv. Pathumthani 1 (PT1). Exogenous GlyBet increased proline concentration in the leaf tissues of water deficit stressed plants depend on a degree of soil water content (SWC), especially at severe water deficit (25% SWC). Foliar GlyBet (100 mM) significantly enhanced chlorophyll a (Chla) and total chlorophyll (TC) content in the leaf tissues of water stressed plants (25% SWC) and stabilized total carotenoids (Cx+c) in 25 and 36% SWC better than those in controlled plant. A positive correlation was observed between Chla and maximum quantum yield of PSII (Fv/Fm), and TC and photon yield of PSII (PSII). In contrast to control plants, where Fv/Fm and PSII and net photosynthetic rate (Pn) declined, Glybet pre-treated plants maintained a high level of Fv/Fm, PSII and Pn even under 25% SWC. Even at the harvest stage, plant height and yield traits such as panicle length and weight, fertility percentage and one-hundred grain weight of GlyBet pre-treated plants were better than those in the control plants when exposed to water stress. The study concludes that exogenous application of GlyBet significantly alleviates water-deficit in indica rice and maintains grain yield.