A prerequisite for genetic modification of cassava is a reliable transformation system. Establishment of a transformation system for cassava will open up the possibility for genetic manipulation and provide the information needed for handling and introduction of genetically modified cassava clones. Methods for efficient cassava transformation, including plant regeneration in vitro, gene delivery, selection of transformed tissues and recovery of transgenic plants, still need to be developed or improved in each cassava cultivar. The two Asian cultivars of cassava; KU 50 (widely grown cultivar with high cyanogens content for non-food purpose) and Hanatee (local cultivar with low cyanogens content for food purpose) were assessed for their ability to produce cyclic somatic embryogenesis from somatic cotyledons. Somatic embryogenesis was induced from different plant materials and various phytohormone concentrations in induction medium and was further developed to the green cotyledons in maturation medium. The developing young green cotyledons could be transferred to organogenesis medium for shoot emerging and plant regeneration. The various kinds of induction media were tested for the most suitable medium which give high cyclic somatic embryogenesis induction frequency in each cassava variety. Cyclic somatic embryogenesis induction frequency were evaluated 60 days after the cotyledons were cultured on each induction medium. The protocol for cyclic somatic embryogenesis induction from different ages of cotyledon had been optimized. The cyclic embryogenesis system can be routinely applied to produce the newly developing young green cotyledons for gene transfer research.