Chemical compositions of roes from three tuna species including skipjack (Katsuwonous pelamis) (SJK), tongol (Thunnus tonggol) (TGL) and bonito (Euthynnus affinis) (BNO) were determined. Tuna roes contained 72.17-73.03% moisture, 18.16-20.15% protein, 3.29-5.68% lipid and 1.79-2.10% ash. Non-defatted and defatted roes from all species contained protein with a molecular weight of 97 kDa as the major component. Glutamic acid and glutamine were the most abundant amino acids (12.18-12.65 g/100 g protein), while K, Na and P were the major elements in defatted-tuna roes. Crude lipids from tuna roes contained phospholipids as the major component (51.22-54.90% of total lipids) with high content of docosahexaenoic acid (C22:6 n-3) (20.53-26.19% of total lipids). Cholesterol contents of crude lipids from the roes of SJK, TGL and BNO were 172, 122 and 94 mg/100 g lipids, respectively. Therefore, tuna roe could be a promising source of valuable nutrients for human food and animal feeds.