Production of six naphthoquinone derivatives, erythrostominone, deoxyerythrostominone, 4-O-methyl erythrostominone, epierythrostominol, deoxyerythrostominol and 3,5,8-trihydroxy-6-methoxy-2-(5-oxohexa-1,3-dienyl)-1,4-naphthoquinone (3,5,8-TMON), were examined during the growth of C. unilateralis BCC 1869 on different carbon and nitrogen sources. The fungus produced erythrostominone more than 50% of total naphtoquinones as a major compound but produced each of other five derivatives less than 20% of total naphthoquinones. The highest volumetric production rate of erythrostominone and overall naphthoquinone production rate were obtained on mannose as a sole carbon source and ammonium sulphate as a sole nitrogen source (4922.4±118.8 mg/L/day and 5.03 g/L/day, respectively). The highest growth rate was obtained on arabinose (0.043 h-1), whereas the maximum overall naphthoquinone concentration was obtained on lactose (2 g/L) at 237 h. These naphthoquinones were produced with no pH controlled and they were first detected at pH around 3-4. These results suggested that carbon and nitrogen had influenced directly the production of naphthoquinones.