This paper reports electrochemical sensing of salbutamol, a prohibited drug in sports, with a new working electrode based on vertically aligned carbon nanotubes (V-CNT). V-CNTs were synthesized by chemical vapor deposition using acetylene and argon gases at 700°C on gold coated silicon substrates. A simple electrochemical cell including V-CNTs, silver wire (Ag) and platinum (Pt) wire was designed. Electrochemical characterization by cyclic voltammetry (CV) was carried out with different salbutamol concentrations ranging from 10 -7 to 10-4 mol · l-1. CV curves exhibited irreversible oxidation peak at ∼0.7 V The current response was linear with sensitivity of 0.13μA/mol-1 and a minimum detection of 3× 10-7 mol l-1. In addition, its pharmaceutical applications were demonstrated. The direct analysis of salbutamol in pharmaceutical products yielded a good analytical feature with wide dynamic working range (0.5 to 100 μM) and matrices' interference was found to be negligible. Thus, V-CNTs electrode is a potential candidate for the electrochemical detection of salbutamol.