In this paper, we present the analysis of the parameters that related to fusion splicing loss of SMF-28 and MP980 optical fiber such as axial misalignment (d) and angular misalignment (θ). Here, the Matching mode field and Phase-front transformer model are used to determine their relationships. We study the effect of d and θ on the fusion splicing loss. It is found that, the increasing of d value is more effective of the fusion splicing loss. Where d values are 0 μm and 5 μm, the fusion splicing loss are 0.02 dB and 3.77 dB, respectively. The parameter θ is also positively correlated with the fusion splicing loss while θ on MP-980 has greater impact than θ on SMF-28 which are 8.36 dB and 8.24 dB respectively for the same θ at 5°. Furthermore, the SMF-28 and MP980 structures for the optimum fusion splicing loss are 4.1 μm and 1.16 μm, respectively. Their optimum fusion splicing loss is 0.02 dB with λ = 1.55 μm while d = 0 μm and θ = 0°. These results can be used to predict the loss caused by single-mode optical fiber fusion splicing, and be applied for the optical communication system. © 2012 IEEE.