Prompt and accurate diagnosis of malaria is the key to prevent disease morbidity and mortality. This study was carried out to evaluate diagnostic performance of 3 commercial rapid detection tests (RDTs), i.e., Malaria Antigen Pf/PanTM, Malaria Ag-PfTM, and Malaria Ag-PvTM tests, in comparison with the microscopic and PCR methods. A total of 460 blood samples microscopically positive for Plasmodium falciparum (211 samples), P. vivax (218), mixed with P. falciparum and P. vivax (30), or P. ovale (1), and 124 samples of healthy subjects or patients with other fever-related infections, were collected. The sensitivities of Malaria Ag-PfTM and Malaria Antigen Pf/PanTM compared with the microscopic method for P. falciparum or P. vivax detection were 97.6% and 99.0%, or 98.6% and 99.0%, respectively. The specificities of Malaria Ag-PfTM, Malaria Ag-PvTM, and Malaria Antigen Pf/PanTM were 93.3%, 98.8%, and 94.4%, respectively. The sensitivities of Malaria Ag-PfTM, Malaria Antigen Pf/PanTM, and microscopic method, when PCR was used as a reference method for P. falciparum or P. vivax detection were 91.8%, 100%, and 96.7%, or 91.9%, 92.6%, and 97.3%, respectively. The specificities of Malaria Ag-PfTM, Malaria Ag-PvTM, Malaria Antigen Pf/PanTM, and microscopic method were 66.2%, 92.7%, 73.9%, and 78.2%, respectively. Results indicated that the diagnostic performances of all the commercial RDTs are satisfactory for application to malaria diagnosis. © 2011, Korean Society for Parasitology.