This study determines what roles non-governmental organizations (NGOs) have played in the formation of the global human rights regime. The addition of transnational actors to the field of International Relations and global human rights had created knowledge gaps which were both theoretical and empirical. Although there was a growing literature in this area, we still did not know very much about the new actors. Moreover, since the old theoretical constructs ignored them, and little conceptual and theoretical work had evolved, we were not certain how to approach these actors. This study has attempted to bridge these gaps in the study of NGOs by addressing itself to both theoretical problem of how to approach the new actors and the need for new data on human rights NGOs. It has attempted to develop an appropriate theoretical framework emphasizing NGO's role in agenda politics of the global regime process, and then using this framework to undertake historical case study research. The study conceptualizes global regime change in terms of "macro" and "micro" processes. The basic "macro" processes characterizing long-term regime change are: regime formation, regime maintenance, and regime transformation. In addition to the "macro" processes of regime change, the framework we present also attempts to conceptualize short-term regime change as "micro" processes involving: agenda politics, negotiation, decision-making, and regime outputs and outcomes. The main proposition of this study is that NGOs will play a significant participant role in the "macro" process of global human rights regime formation from 1945 to 1954. More specifically, human rights NGOs' role will be significant in the agenda-politics phase of the "micro" regime processes. The findings indicates that NGOs did play an important role in the agenda politics of global human rights regime formation. It has shown how NGOs helped form the present global human rights substantive regime component, the procedural-organizational regime component, and the implementation mechanisms.