| ชื่อเรื่อง | : | Analysis of N-glycosylated protein and N-glycans in human plasma by hydrophilic enrichment strategy |
| นักวิจัย | : | Ma, Cheng , Pan, Yiting , Zhang, Qi , Wang, Jifeng , Qian, Xiaohong , Ying, Wantao |
| คำค้น | : | DRNTU::Science::Physics , DRNTU::Science::Biological sciences |
| หน่วยงาน | : | Nanyang Technological University, Singapore |
| ผู้ร่วมงาน | : | - |
| ปีพิมพ์ | : | 2556 |
| อ้างอิง | : | Ma, C., Pan, Y., Zhang, Q., Wang J., Qian, X., & Ying, W. (2013). Analysis of N-glycosylated protein and N-glycans in human plasma by hydrophilic enrichment strategy. Chinese journal of chromatography, 31(11), 1057-1063. , 1872-2059 , http://hdl.handle.net/10220/25055 , http://www.chrom-china.com/EN/abstract/abstract13508.shtml |
| ที่มา | : | - |
| ความเชี่ยวชาญ | : | - |
| ความสัมพันธ์ | : | Chinese journal of chromatography |
| ขอบเขตของเนื้อหา | : | - |
| บทคัดย่อ/คำอธิบาย | : | N-glycosylation of proteins is one of the most important post-translational modifications (PTM). Many diagnostic biomarkers and therapeutic targets are glycosylated proteins. However, it is still a big challenge to identify glycoproteins in human plasma, because of the high dynamic range and microheterogeneity of glycosylation. In this work, hydrophilic interaction liquid chromatography (HILIC) enrichment, high-pH reversed-phase prefractionation and high accurate mass spectrometry (MS) analysis were combined to profile the N-glycoproteins in human plasma. In total, 637 N-glycosites from 299 glycoproteins (protein groups) were identified. There were also 31 glycoforms recognized after HILIC enrichment and MS analysis. Among the results, 107 glycosylation sites (16.8%) are newly found. This study provided a simple and reliable strategy for exploring potential N-glycoprotein biomarkers from complicated biological systems. |
| บรรณานุกรม | : |
Ma, Cheng , Pan, Yiting , Zhang, Qi , Wang, Jifeng , Qian, Xiaohong , Ying, Wantao . (2556). Analysis of N-glycosylated protein and N-glycans in human plasma by hydrophilic enrichment strategy.
กรุงเทพมหานคร : Nanyang Technological University, Singapore. Ma, Cheng , Pan, Yiting , Zhang, Qi , Wang, Jifeng , Qian, Xiaohong , Ying, Wantao . 2556. "Analysis of N-glycosylated protein and N-glycans in human plasma by hydrophilic enrichment strategy".
กรุงเทพมหานคร : Nanyang Technological University, Singapore. Ma, Cheng , Pan, Yiting , Zhang, Qi , Wang, Jifeng , Qian, Xiaohong , Ying, Wantao . "Analysis of N-glycosylated protein and N-glycans in human plasma by hydrophilic enrichment strategy."
กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2556. Print. Ma, Cheng , Pan, Yiting , Zhang, Qi , Wang, Jifeng , Qian, Xiaohong , Ying, Wantao . Analysis of N-glycosylated protein and N-glycans in human plasma by hydrophilic enrichment strategy. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2556.
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