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In situ synthesis of protein-resistant poly(oligo(ethylene glycol)methacrylate) films in capillary for protein separation

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : In situ synthesis of protein-resistant poly(oligo(ethylene glycol)methacrylate) films in capillary for protein separation
นักวิจัย : Huang, Rong , Ferhan, Abdul Rahim , Guo, Longhua , Qiu, Bin , Lin, Zhenyu , Kim, Dong-Hwan , Chen, Guonan
คำค้น : DRNTU::Engineering::Bioengineering
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2557
อ้างอิง : Huang, R., Ferhan, A. R., Guo, L., Qiu, B., Lin, Z., Kim, D. H., et al. (2014). In situ synthesis of protein-resistant poly(oligo(ethylene glycol)methacrylate) films in capillary for protein separation. RSC advances, 4(10), 4883-. , 2046-2069 , http://hdl.handle.net/10220/20971 , http://dx.doi.org/10.1039/c3ra47012a
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : RSC advances
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

We report a method to modify a silica capillary with poly(oligo(ethylene glycol)methacrylate) (POEGMA) and its performance for protein separation. We optimized the grafting density and thickness of the POEGMA film and investigated the effect of running buffer pH, ionic strength, separation voltage, and sample injection time. The POEGMA-coated capillary was successfully utilized for the separation of six model proteins. Under optimized conditions, the resolution was found to be much better than a bare capillary, and even better than a widely used, commercially available PEG-modified capillary.

บรรณานุกรม :
Huang, Rong , Ferhan, Abdul Rahim , Guo, Longhua , Qiu, Bin , Lin, Zhenyu , Kim, Dong-Hwan , Chen, Guonan . (2557). In situ synthesis of protein-resistant poly(oligo(ethylene glycol)methacrylate) films in capillary for protein separation.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Huang, Rong , Ferhan, Abdul Rahim , Guo, Longhua , Qiu, Bin , Lin, Zhenyu , Kim, Dong-Hwan , Chen, Guonan . 2557. "In situ synthesis of protein-resistant poly(oligo(ethylene glycol)methacrylate) films in capillary for protein separation".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Huang, Rong , Ferhan, Abdul Rahim , Guo, Longhua , Qiu, Bin , Lin, Zhenyu , Kim, Dong-Hwan , Chen, Guonan . "In situ synthesis of protein-resistant poly(oligo(ethylene glycol)methacrylate) films in capillary for protein separation."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2557. Print.
Huang, Rong , Ferhan, Abdul Rahim , Guo, Longhua , Qiu, Bin , Lin, Zhenyu , Kim, Dong-Hwan , Chen, Guonan . In situ synthesis of protein-resistant poly(oligo(ethylene glycol)methacrylate) films in capillary for protein separation. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2557.