| ชื่อเรื่อง | : | Improving signal-to-noise ratio of structured light microscopy based on photon reassignment |
| นักวิจัย | : | Singh, Vijay Raj , Choi, Heejin , Yew, Elijah Y. S. , Bhattacharya, Dipanjan , Yuan, Luo , Sheppard, Colin J. R. , Rajapakse, Jagath C. , Barbastathis, George , So, Peter T. C. |
| คำค้น | : | - |
| หน่วยงาน | : | Nanyang Technological University, Singapore |
| ผู้ร่วมงาน | : | - |
| ปีพิมพ์ | : | 2554 |
| อ้างอิง | : | Singh, V. R., Choi, H., Yew, E. Y. S., Bhattacharya, D., Yuan, L., Sheppard, C. J. R., et al. (2012). Improving signal-to-noise ratio of structured light microscopy based on photon reassignment. Biomedical Optics Express, 3(1), 206-214. , http://hdl.handle.net/10220/10165 , http://dx.doi.org/10.1364/BOE.3.000206 |
| ที่มา | : | - |
| ความเชี่ยวชาญ | : | - |
| ความสัมพันธ์ | : | Biomedical optics express |
| ขอบเขตของเนื้อหา | : | - |
| บทคัดย่อ/คำอธิบาย | : | In this paper, we report a method for 3D visualization of a biological specimen utilizing a structured light wide-field microscopic imaging system. This method improves on existing structured light imaging modalities by reassigning fluorescence photons generated from off-focal plane excitation, improving in-focus signal strength. Utilizing a maximum likelihood approach, we identify the most likely fluorophore distribution in 3D that will produce the observed image stacks under structured and uniform illumination using an iterative maximization algorithm. Our results show the optical sectioning capability of tissue specimens while mostly preserving image stack photon count, which is usually not achievable with other existing structured light imaging methods. |
| บรรณานุกรม | : |
Singh, Vijay Raj , Choi, Heejin , Yew, Elijah Y. S. , Bhattacharya, Dipanjan , Yuan, Luo , Sheppard, Colin J. R. , Rajapakse, Jagath C. , Barbastathis, George , So, Peter T. C. . (2554). Improving signal-to-noise ratio of structured light microscopy based on photon reassignment.
กรุงเทพมหานคร : Nanyang Technological University, Singapore. Singh, Vijay Raj , Choi, Heejin , Yew, Elijah Y. S. , Bhattacharya, Dipanjan , Yuan, Luo , Sheppard, Colin J. R. , Rajapakse, Jagath C. , Barbastathis, George , So, Peter T. C. . 2554. "Improving signal-to-noise ratio of structured light microscopy based on photon reassignment".
กรุงเทพมหานคร : Nanyang Technological University, Singapore. Singh, Vijay Raj , Choi, Heejin , Yew, Elijah Y. S. , Bhattacharya, Dipanjan , Yuan, Luo , Sheppard, Colin J. R. , Rajapakse, Jagath C. , Barbastathis, George , So, Peter T. C. . "Improving signal-to-noise ratio of structured light microscopy based on photon reassignment."
กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2554. Print. Singh, Vijay Raj , Choi, Heejin , Yew, Elijah Y. S. , Bhattacharya, Dipanjan , Yuan, Luo , Sheppard, Colin J. R. , Rajapakse, Jagath C. , Barbastathis, George , So, Peter T. C. . Improving signal-to-noise ratio of structured light microscopy based on photon reassignment. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2554.
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