| ชื่อเรื่อง | : | Purification and characterization of Alu I methylase |
| นักวิจัย | : | Yoon, Ho Sup , Suh, Hyang , Han, Moon H. , Yoo, Ook Joon |
| คำค้น | : | DRNTU::Science::Biological sciences. |
| หน่วยงาน | : | Nanyang Technological University, Singapore |
| ผู้ร่วมงาน | : | - |
| ปีพิมพ์ | : | 2528 |
| อ้างอิง | : | Yoon, H. S., Suh, H., Han, M. H., & Yoo, O. J. (1985). Purification and characterization of Alu I methylase. Korean Biochemistry Journal., 18(1), 82-87. , http://hdl.handle.net/10220/8716 , http://www.jbmb.or.kr/jbmbonline/18_1/list.html |
| ที่มา | : | - |
| ความเชี่ยวชาญ | : | - |
| ความสัมพันธ์ | : | Korean biochemistry journal |
| ขอบเขตของเนื้อหา | : | - |
| บทคัดย่อ/คำอธิบาย | : | Alu I methylase has been isolated from 300g (wet weight) cells of Arthrobacter luteus. After ammonium sulfate fractionation, the protein which has methylase activity was purified through phosphocellulose, DEAE-cellulose, Heparin agarose, and Hydroxylapatite column chromatography. The methylated DNA by the purified methylase was resistatnt against Alu I endonuclease. The purified Alu I methylase was essentially homogeneous as judged by 10% SDS-polyacrylamide gel electrophoresis, and the apparent subunit molecular weight was 56,000±1,000. The specific activity of the enzyme was 1.32 × 105 units per mg protein. |
| บรรณานุกรม | : |
Yoon, Ho Sup , Suh, Hyang , Han, Moon H. , Yoo, Ook Joon . (2528). Purification and characterization of Alu I methylase.
กรุงเทพมหานคร : Nanyang Technological University, Singapore. Yoon, Ho Sup , Suh, Hyang , Han, Moon H. , Yoo, Ook Joon . 2528. "Purification and characterization of Alu I methylase".
กรุงเทพมหานคร : Nanyang Technological University, Singapore. Yoon, Ho Sup , Suh, Hyang , Han, Moon H. , Yoo, Ook Joon . "Purification and characterization of Alu I methylase."
กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2528. Print. Yoon, Ho Sup , Suh, Hyang , Han, Moon H. , Yoo, Ook Joon . Purification and characterization of Alu I methylase. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2528.
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