| ชื่อเรื่อง | : | The specificity and catalytic properties of Alu I methylase |
| นักวิจัย | : | Yoon, Ho Sup , Suh, Hyang , Kim, Kitae , Han, Moon H. , Yoo, Ook Joon |
| คำค้น | : | DRNTU::Science::Biological sciences. |
| หน่วยงาน | : | Nanyang Technological University, Singapore |
| ผู้ร่วมงาน | : | - |
| ปีพิมพ์ | : | 2528 |
| อ้างอิง | : | Yoon, H. S., Suh, H., Kim, K., Han, M. H., & Yoo, O. J. (1985). The specificity and catalytic properties of Alu I methylase. Korean Biochem. J.,18(1), 88-93. , http://hdl.handle.net/10220/8714 , http://www.jbmb.or.kr/jbmbonline/18_1/list.html |
| ที่มา | : | - |
| ความเชี่ยวชาญ | : | - |
| ความสัมพันธ์ | : | Korean biochemistry journal |
| ขอบเขตของเนื้อหา | : | - |
| บทคัดย่อ/คำอธิบาย | : | The specific methylation site for Alu I methylase was the cytosine nucleotide in Alu I sequence. The position of the methylated cytosine nucleotide was determined by the chemical cleavage reactions of the Maxam-Gilbert DNA sequencing procedure. As expected, the methylated cytosine nucleotide bands were disappeared on C+ T and C lanes on 12% sequencing gels. Alu I methylase was maximally active at near pH 7.5 in the presence of 50 mM NaCl. The methylase did not require Mg++ for activity, and obeyed Michaelis-Menten Kinetics with respect to both AdoMet and DNA. At 37°C, the Km for AdoMet was 0.44 μM, that for the Alu I site of pBR 322 DNA was 4.03 nM, and the corresponding turnover numbers were 1.83 methyl transfer per minute per monomer and 1.61 transfers per minute per monomer, respectively. |
| บรรณานุกรม | : |
Yoon, Ho Sup , Suh, Hyang , Kim, Kitae , Han, Moon H. , Yoo, Ook Joon . (2528). The specificity and catalytic properties of Alu I methylase.
กรุงเทพมหานคร : Nanyang Technological University, Singapore. Yoon, Ho Sup , Suh, Hyang , Kim, Kitae , Han, Moon H. , Yoo, Ook Joon . 2528. "The specificity and catalytic properties of Alu I methylase".
กรุงเทพมหานคร : Nanyang Technological University, Singapore. Yoon, Ho Sup , Suh, Hyang , Kim, Kitae , Han, Moon H. , Yoo, Ook Joon . "The specificity and catalytic properties of Alu I methylase."
กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2528. Print. Yoon, Ho Sup , Suh, Hyang , Kim, Kitae , Han, Moon H. , Yoo, Ook Joon . The specificity and catalytic properties of Alu I methylase. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2528.
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