| ชื่อเรื่อง | : | Cloning, expression and characterization of the human transcription elongation factor, TFIIS |
| นักวิจัย | : | Yoo, Ook Joon , Yoon, Ho Sup , Baek, Kwanghee , Jeon, Choon Ju , Miyamoto, Kenichi , Ueno, Akemichi , Agarwal, Kan |
| คำค้น | : | DRNTU::Science::Biological sciences::Biochemistry. |
| หน่วยงาน | : | Nanyang Technological University, Singapore |
| ผู้ร่วมงาน | : | - |
| ปีพิมพ์ | : | 2534 |
| อ้างอิง | : | Yoo, O. J., Yoon, H. S., Baek, K., Jeon, C. J., Miyamoto, K., Ueno, A., & Agarwal, K. (1991). Cloning, expression and characterization of the human transcription elongation factor, TFIIS. Nucleic acids research, 19(5), 1073-1079. , http://hdl.handle.net/10220/7658 , http://dx.doi.org/10.1093/nar/19.5.1073 |
| ที่มา | : | - |
| ความเชี่ยวชาญ | : | - |
| ความสัมพันธ์ | : | Nucleic acids research |
| ขอบเขตของเนื้อหา | : | - |
| บทคัดย่อ/คำอธิบาย | : | The cDNA for the human elongation factor, TFIIS, has been cloned and expressed in E. coil with the T7 expression system. This 280-amino acid TFIIS protein is shorter by 21 residues than that of the mouse. The missing 21 residues are located in the amino-terminal region, which is not thought to be required for transcriptional stimulation. Apart from this gap, human and mouse proteins reveal 96% overall identity and 98.5% sequence similarity If conservative substitutions are taken into account. The bacterially expressed human protein and the purified calf thymus proteins are indistinguishable in their ability to stimulate transcript elongation by purified RNA polymerase II. Estimation of the native molecular size of the human protein in solution indicates that It exists as a dimer. |
| บรรณานุกรม | : |
Yoo, Ook Joon , Yoon, Ho Sup , Baek, Kwanghee , Jeon, Choon Ju , Miyamoto, Kenichi , Ueno, Akemichi , Agarwal, Kan . (2534). Cloning, expression and characterization of the human transcription elongation factor, TFIIS.
กรุงเทพมหานคร : Nanyang Technological University, Singapore. Yoo, Ook Joon , Yoon, Ho Sup , Baek, Kwanghee , Jeon, Choon Ju , Miyamoto, Kenichi , Ueno, Akemichi , Agarwal, Kan . 2534. "Cloning, expression and characterization of the human transcription elongation factor, TFIIS".
กรุงเทพมหานคร : Nanyang Technological University, Singapore. Yoo, Ook Joon , Yoon, Ho Sup , Baek, Kwanghee , Jeon, Choon Ju , Miyamoto, Kenichi , Ueno, Akemichi , Agarwal, Kan . "Cloning, expression and characterization of the human transcription elongation factor, TFIIS."
กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2534. Print. Yoo, Ook Joon , Yoon, Ho Sup , Baek, Kwanghee , Jeon, Choon Ju , Miyamoto, Kenichi , Ueno, Akemichi , Agarwal, Kan . Cloning, expression and characterization of the human transcription elongation factor, TFIIS. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2534.
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