To investigate the viability and normality after freezing and thawing of bovine embryos produced in vitro, we have accomplished the procedures to mature oocytes (in TCM-199 medium) and fertilize in vitro with heparin treated sperm (in TALP-glucose free medium).The oviductal epithelial cells were co-cultured in vitro (in TCM-199 + 10% HTFCS) and were capable of supporting normal growth of embryos to the stages at which non-surgical embryo transfer could be performed (compact morula, early blastocyst and blastocyst). Oocytes with intact cumulus cells showed higher (P

0.375 M glycerol and 0.3 M sucrose

and 0.3 M sucrose) reported by other investigators (50% VS 35% in compact morula

75% VS 41% in early blastocyst

33% VS 33% in blastocyst). In vitro testing, the competency of embryonic development to an advanced stage was higher in blastocyst than those from compact morula or early blastocyst. The result indicated that bovine embryos produced in vitro could be frozen and thawed similar to those obtained from in vivo procedures. The survival rate by non-surgical transferring to recipients was better by using single step in freezing and thawing, and early blastocysts were the best survivors.