Skeletonema preserved with four different methods: sun-dried, heat dried, refrigerated and freezed were tested as larval feed for tiger prawn (Penaeus monodon fabricius) larvae, comparing to live Skeletonema. Tiger prawn larvae fed with refrigerated Skeletonema were able to develop into post larva stage. When 1-day refrigerated Skeletonema were used, average survival rate of tiger prawn larvae were 91.42, 85.20, 83.42 and 81.75 percents for protozoea 3, mysis 1, mysis 3 and post larva 1, respectively. There were no significant different (P>.05) between average survival rate of larvae fed with 1-day refrigerated Skeletonema and larvae fed with live Skeletonema. Shrimp larvae were unable to develop into mysis 2 stage when they were fed with sun-dried and heat-dried Skeletonema but shrimp larvae fed with freezed Skeletonema were able to develop into mysis 1. Longer preservation period result in lower quality of preserved Skeletonema indicated by lower survival rate of experimental shrimp larvae.