Thesis (M.Sc.)--Chulalongkorn University, 2009

The activity of Glycosmis prava extracts were investigated in LPS-treated macrophage J77A.1 cell. It was found that the fractions of branches and leaves obtained from hexane, ethyl acetate suppressed nitric oxide production without cytotoxicity in the study dose (G1, G2, G5 and G6). Their IC50 for NO production varied from 11.12 – 44.70 µg/ml. The RT-PCR showed that G. parva fractions significantly inhibited pro-inflammatory expression of cytokines, TNF-α, IL-1β and IL-6 in different manner. The prominent effect on TNF-α generation were found in ethyl acetate and hexane extract from leaves (G5 and G6) which caused about 94.2% and 95% at the concentration of 25 respectively. For IL-1β expression, G2 at the concentration of 20 µg/ml demonstrated highest inhibition effect (69.8%). However, inhibition effect on IL-6 expression were not evidenced in G2 and G5 from this present study. Moreover the expression of COX-2 and iNOS mRNA were also prohibited by G. parva extract (G1, G2, G5 and G6). The greatest inhibition of iNOS expression was seen in G6 at 25 µg/ml concentration (82.5%). The expression of COX-2 was pronounced inhibited by G6 at 25 µg/ml concentration (93%).The results obtained from this study indicated the potential of G. parva in down regulation of pro-inflammatory cytokines and enzymes production which may consider them as the leading compounds for anti-inflammatory action.