Curcumin (CM) is one such natural therapeutic agent which derived from turmeric (Curcumin longa Linn.). CM has been demonstrated safety and efficacy. However, it has poor oral bioavailability due to its extremely low aqueous solubility, rapid systemic elimination, inadequate tissue absorption, and degradation at alkaline pH. Among the different drug delivery systems (DDS) available, the biodegradable pH responsive polymeric micelles (PMs) have shown immense potential for oral delivery of poorly water-soluble drugs. In this study, novel pH responsive nanoPMs were developed based on an introduction of hydrophobic and hydrophilic moieties into a chitosan backbone. The particle sizes and zeta-potentials of nanoPMs in water with and without CM were found to be around 100 nm in diameter and -30 mV, respectively. The morphologies of nanoPMs before and after loaded CM showed spherical shape, investigating by TEM technique (Figure 1). The released profiles of CM in PBS buffer showed sustained release at pH 6.8 and 7.4, while small amount of CM was released at pH 1.2. The cellular uptake of fluorescent-nanoPMs in Hela, SiHa, and C33a cells was increased with increasing incubation time from 6 h to 24 h. The CM-loaded nanoPMs enhanced aqueous solubility, and also enhanced anticancer activity by decreasing IC50 values lower than 8 times and induction of early apoptosis against all cervical cancer cells than free CM. Moreover, the CM-loaded nanoPMs can be made in a solid dosage form to improve stability of CM. In our DDS, it can be applied in oral curcumin delivery system for anticervical cancer cells