Thesis (M.Sc.)--Chulalongkorn University, 2007

In this study we isolated bioactive compounds from the endophytic fungus isolate SM1 from a bark of Suregada multiflora (A. Juss.) Baill. Crude extract from the YES culture broth of endophytic fungus isolate SM1 was purified by chromatographic techniques to afford two compounds, which were identified as 2-phenylethanol, and 4-methyl-5-oxo-2-propyl-tetrahydro-furan-3-carboxylic acid. The chemical structures of isolated compounds were elucidated through extensive analyses of UV, IR, MS, and NMR spectroscopic data and by comparison with literature. The isolated compounds showed no antimicrobial activity, antimalarial activity, and anticancer activity. Based on morphology and nucleotide sequence of ITS1-5.8S-ITS2 regions of rDNA, the endophytic fungus isolate SM1 was identified as Lasiodiplodia sp., possibly an anamorph of Botryosphaeria rhodina (100 % sequence homology).