Nowaday, the problem of fungi and bacteria was found in natural rubber (NR) products. This research work studied the processing efficiency of antifungal and bacterial natural rubber filled with silver and copper nanoparticles in the restroom application. This work was used silver nanoparticles (average size 10-60 nm), copper nanoparticles (average size 30-60 nm) and tested for infection with four types namely E. coli, S. aureus, A. flavus and Penicillium sp.. It was found that using 100:100 ppm of silver and copper nanoparticles have more effective in bacterial (E. coli and S. aureus) and fungi (A. flavus and Penicillium sp.). In contrast, aging and mechanical properties are investigated. The results showed an increase of tensile strength with 100:100 ppm of silver and copper nanoparticles. Elongation at yield are exhibited not different as much in all compositions. It could be concluded that the best ratio of silver and copper nanoparticles was 100:100 ppm for antifungi and bacterial natural rubber products.