In this study chlorophyll measurements were made during March 2012 in the estuarine waters of Off Kakinada and Yanam coast, Bay of Bengal onboard a coastal vessel. In-situ water samples and optical data was collected at 21 stations (surface to 150 m depth) using Underwater radiometer (Hyperpro-II). In-vivo chlorophyll profiles were collected using wet labs fluorometer integrated with underwater Hyperspectral radiometer. Chlorophyll-a concentrations were estimated using HPLC by collecting the water samples at each sampling location. And also chlorophyll-a concentrations were retrieved from the OCM-2 data of OCEANSAT-2 satellite, processed using SeaDAS v.6.2 with the available global ocean colour algorithms namely, OC2 and OC4V4. A total of 33 samples used covering all the stations for chlorophyll-a estimation, and surface water samples of all the stations only being used for direct comparison among chlorophyll concentrations of HPLC, in-situ (fluorometrically integrated to Hyperpro-II) and retrieved from OCM-2. A good correlation found between the Fluorometer derived and HPLC measured chlorophyll-a concentration with an R2 value of 0.78. The relation between Chlorophyll-a concentration measured from HPLC and retrieved from OCM-2 (OC2 and OC4V4 algorithms) using SeaDASv.6.2 for 10 samples has been compared for validation and obtained an R2 value of 0.6. Also comparisons done with the in-situ measured (fluorometer) Chlorophyll-a concentration with OCM-2 chlorophyll data (OC4-V4 and OC2 algorithms) and validation with 10 concurrent in-situ surface measurements showed a significant overestimation by OCM-2 at low chlorophyll-a concentrations and underestimation at high chlorophyll-a concentrations.