The preparation conditions by chemical agents i.e. phosphoric acid, potassium hydroxide,and zinc chloride of activated carbon from jatropha oil waste were determined at varioustemperatures (500, 600, and 700oC), activation times (60, 120, and 180 min.), and ratios of rawmaterial:chemical agent (1:0.5 y 1:2). The activations by physical agents i.e. steam and carbon dioxide at various temperatures (450 - 1,000oC) and activating time (45 min. y 2 hr.) were also determined. Adsorption isotherm of the maximum surface area of each activation method was revealed. Finally, desorption efficiency via hydrochloric acid, nitric acid, acetic acid, EDTA, sodium chloride and sodium hydroxide solutions at various concentration, temperature and time was performed. The results found that the best chemical activation condition for the production in term of high surface area of activated carbon from jatropha seed cake occurred with potassium hydroxide at the activation temperature of 700oC, activation time of 180 minutes, and impregnation ratio of 1:0.75. At this optimal condition, the BET surface area was 666 m2/g activated carbon. However, highest net BET surface area 287 m2/g raw material occurred from zinc chloride agent at temperature of 700oC, activation time of 120 minutes, and impregnation ratio of 1:0.75. The structure of activated carbon were mainly amorphous, where the activated carbon with zinc chloride might contain the crystals of ZnO and Zn5(OH)8Cl2 .H2O on the carbon surface. The surface of activated carbon might contain several functional groups including aromatic, benzene derivatives, and hydroxyl groups. Net surface area provided by zinc chloride shown the best at 289 m2/g raw material. For physical activation, steam activation found the maximum surface area and net surface area at 533 m2/g activated carbon and 288 m2/g raw material, respectively, at the activation temperature of 950oC and activation time of 60 minutes. Both Freundlich and Langmuir isotherms were best fitted with this adsorption at pH 3. The maximum desorption efficiency of 86.22% was derived from EDTA solution at concentration of 0.1 mol, time of 60 minutes and temperature of 35 oC