Burkholderia pseudomallei is a causative agent of melioidosis, a severe and fatal human infectious disease which is endemic in Southeast Asia and Northern Australia. In Thailand, 2,000 to 3,000 new cases are diagnosed every year. The incidence of clinical melioidosis is estimated to be 3.6-5.5 cases per 100,000 per annum in the northeastern part of Thailand. The infection is primarily acquired via the inoculation and inhalation. The most severe form of melioidosis leads to death in approximately 40-50% of patients in Thailand despite optimal antibiotic therapy. The main objective of this study is to investigate a B. pseudomallei virulence factor called BimA (Burkholderia Intracellular Motility) and the proteomic profiling of B. pseudomallei under salt stress. BimA protein was identified as a bacterial virulence factor involved in actin-based motility of B. pseudomallei inside host cell. Data obtained from this study has important implications for the design and reliability of bimA based tests to differentiate between Burkholderia species and highlight the existence of polymorphisms that have the potential to influence actin binding and assembly for B. pseudomallei intercellular spread and virulence. For proteomic study, we report the first dataset of the secretome of B. pseudomallei and its alterations during salt stress. These findings may provide some novel insights into the adaptive response of this microorganism to survive during the salt stress. Further characterizations and functional analyses of these altered proteins may lead to identification of new therapeutic targets or vaccine development.