The quantities and qualities of proteins in extracts from 12 glove brands of four glove types, powdered latex examination gloves, powder-free latex examination gloves, powdered latex surgical gloves/ and synthetic (vinyl) gloves, were determined and compared. All glove extracts were measured for protein concentrations by Modified Lowry assay and molecular weights of proteins were identified by use of not only Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) but also High Performance Liquid Chromatography (HPLC) and Liquid Chromatography/Mass Spectrometry/Mass Spectrometry (LC/MS/MS) and Matrix Assisted Laser Desorption Ionization (MALDI)/Time-Of-Flight (TOF)/ Time-Of-Flight (TOF) The results showed various quantities and qualities of proteins in tested gloves. The powdered latex examination gloves had the highest median of protein concentration (271.67ug/g), followed by powder-free latex examination (87.20ug/g), powdered latex surgical gloves (54.15 ug/g) and synthetic (vinyl) gloves (54.10 ug/g), respectively. Significant differences of protein concentration were found among the glove types. (p<0.01) The quantitative and qualitative study of protein by HPLC method identified amino acid content. The total protein is equal to total amount of amino acid. In this study identified 31 standard amino acids content and calculated in %amino acid. 31 standard amino acid contents are represented as Creatine, Phosphoserine, Aspatic acid, Glutamic acid, Alpha Aminoadipic acid, Hydroxyproline, Serine, Asparagine, Glycine, Glutamine Taurine, Histidine, gramma Aminobytyric acid, Citrulline, Theonine, Alanine, beta Aminobytyric acid, Carnosine, Arginne, Methionine Sulfone, Proline, alpha Aminobytyric acid, Tyrosine, Valine, Methionine, Isoleucine, Leucine, Phenylalanine, Tryptophan, Ornithine and Lysine. The results of powdered latex examination gloves showed that had %amino acid from high to less in the first 7 order follow by Citrulline, Alanine, Glycine, Threonine, Alpha Aminoadipic acid, Methionine Sulfone and Creatine. The results of powder-free latex examination gloves showed that had %amino acid from high to less in the first 7 order follow by Citrulline, Threonine, Alanine, Creatine, Methionine Sulfone, Alpha Aminoadipic acid and Lysine. The results of powdered latex surgical gloves showed that had %amino acid from high to less in the first 7 order follow by Citrulline, Threonine, Alanine, Methionine Sulfone, Alpha Aminoadipic acid, Creatine, and Lysine. The results of synthetic (vinyl) gloves showed that had %amino acid from high to less in the first 7 order follow by Citrulline, Threonine, Methionine Sulfone, Alpha Aminoadipic acid, Alanine, Creatine, and Methionine. The qualitative study by SDS-PAGE method identified proteins at molecular weights of <10, 11, 12, 15, 25, 30 45, 60, 65, 70, and 100 kd. The powdered latex examination gloves contained proteins at molecular weights of 11, 15, 25, 30_ and 45 kd, while powder free latex examination gloves contained proteins at molecular weights of 11, 15, 25, 30_ and 100 kd. The powdered latex surgical gloves contained protein at molecular weights of <10 11, 12, 60, 65, and 70 kd. The synthetic (vinyl) gloves contained protein at molecular weights of <10, 11, 12, and 70 kd. The most common protein had a at molecular weights of 11 kd. The qualitative determination by LC/MS/MS and MALDI/TOF/TOF method showed that the powdered latex examination gloves and the powdered latex surgical gloves contained proteins at molecular weight of 22 kd, while powder free latex examination gloves contained proteins at molecular weights of 22 and 66 kd. For the synthetic (vinyl) gloves found no containing protein caused allergic reactions. All latex gloves in this study had proteins, which may induce allergic reactions. Therefore, individuals who are definitively diagnosed as latex protein hypersensitivity should avoid using latex gloves.