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Opisthorchis viverrini: Detection by polymerase chain reaction (PCR) in human stool samples

หน่วยงาน National Institute Of Oceanography (NIO), India

รายละเอียด

ชื่อเรื่อง : Opisthorchis viverrini: Detection by polymerase chain reaction (PCR) in human stool samples
นักวิจัย : Umesha, K.R. , SanathKumar , Parvathi, A. , Duenngai, K. , Sithithaworn, P. , Karunasagar, Indrani , Karunasagar, Iddya
คำค้น : microorganisms , polymerase chain reaction , health and safety , defection
หน่วยงาน : National Institute Of Oceanography (NIO), India
ผู้ร่วมงาน : -
ปีพิมพ์ : 2551
อ้างอิง : Experimental Parasitology, vol.120; 353-356p. , http://drs.nio.org/drs/handle/2264/1495
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : -
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

A polymerase chain reaction (PCR) assay was evaluated for detection of Opisthorchis viverrini eggs in the stool specimens of light and heavily infected individuals in Khon Kaen province of Thailand. A total of 75 fecal specimens were analyzed by PCR following DNA extraction. All the microscopically positive samples were positive by PCR, while 23 of 30 (76.6%) microscopically negative samples were also PCR positive. The sensitivity of the assay was 5 eggs/g of stool. This method is potentially useful in the diagnosis of human opisthorchiasis in endemic areas for treatment and in epidemiological investigations.

บรรณานุกรม :
Umesha, K.R. , SanathKumar , Parvathi, A. , Duenngai, K. , Sithithaworn, P. , Karunasagar, Indrani , Karunasagar, Iddya . (2551). Opisthorchis viverrini: Detection by polymerase chain reaction (PCR) in human stool samples.
    : National Institute Of Oceanography (NIO), India.
Umesha, K.R. , SanathKumar , Parvathi, A. , Duenngai, K. , Sithithaworn, P. , Karunasagar, Indrani , Karunasagar, Iddya . 2551. "Opisthorchis viverrini: Detection by polymerase chain reaction (PCR) in human stool samples".
    : National Institute Of Oceanography (NIO), India.
Umesha, K.R. , SanathKumar , Parvathi, A. , Duenngai, K. , Sithithaworn, P. , Karunasagar, Indrani , Karunasagar, Iddya . "Opisthorchis viverrini: Detection by polymerase chain reaction (PCR) in human stool samples."
    : National Institute Of Oceanography (NIO), India, 2551. Print.
Umesha, K.R. , SanathKumar , Parvathi, A. , Duenngai, K. , Sithithaworn, P. , Karunasagar, Indrani , Karunasagar, Iddya . Opisthorchis viverrini: Detection by polymerase chain reaction (PCR) in human stool samples. : National Institute Of Oceanography (NIO), India; 2551.