| ชื่อเรื่อง | : | Cell damage and recovery in cryopreserved microphytobenthic diatoms |
| นักวิจัย | : | Mitbavkar, S. , Anil, A.C. |
| คำค้น | : | phytoplankton culture , cell culture , freezing storage , cryobiology , bilogical damage , intertidal environment , Amphora coffeaeformis , Navicula delicatula |
| หน่วยงาน | : | National Institute Of Oceanography (NIO), India |
| ผู้ร่วมงาน | : | - |
| ปีพิมพ์ | : | 2549 |
| อ้างอิง | : | Cryobiology, Vol.53; 143-147p. , http://drs.nio.org/drs/handle/2264/815 |
| ที่มา | : | - |
| ความเชี่ยวชาญ | : | - |
| ความสัมพันธ์ | : | - |
| ขอบเขตของเนื้อหา | : | - |
| บทคัดย่อ/คำอธิบาย | : | Two pennate microphytobenthic diatoms, Amphora coffeaeformis (Agardh) Kutzing and Navicula transitans var. derasa f. delicatula Heimdal, were cryopreserved and monitored on thawing to track the mechanical injuries and their post-preservation recovery. Cells were subjected to (1) direct freezing in liquid nitrogen and (2) two-step cooling with and without the cryoprotectant, dimethyl sulfoxide (Me2 SO). Mechanical injury due to exposure to low temperature diVered between the two species. While A. coffeaeformis cells were intact and could survive even direct freezing without a cryoprotectant, N. delicatula cell chloroplasts were damaged. However, the two-step cooling along with a cryoprotectant minimized the mechanical injury to cells of both species thereby enhancing the post-thaw viability. |
| บรรณานุกรม | : |
Mitbavkar, S. , Anil, A.C. . (2549). Cell damage and recovery in cryopreserved microphytobenthic diatoms.
: National Institute Of Oceanography (NIO), India. Mitbavkar, S. , Anil, A.C. . 2549. "Cell damage and recovery in cryopreserved microphytobenthic diatoms".
: National Institute Of Oceanography (NIO), India. Mitbavkar, S. , Anil, A.C. . "Cell damage and recovery in cryopreserved microphytobenthic diatoms."
: National Institute Of Oceanography (NIO), India, 2549. Print. Mitbavkar, S. , Anil, A.C. . Cell damage and recovery in cryopreserved microphytobenthic diatoms. : National Institute Of Oceanography (NIO), India; 2549.
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