Polymerase Chain Reaction based Short Tandem Repeat (PCR-STR) method provides an unprecedented DNA profiling technique for forensic community in identification and individualization purposes. In the analysis of biological material using DNA profiling technique, the information regarding the distribution of allele frequency in a defmed population is mandatory. This particularly necessary in paternity testing that involves compilation of Paternity Index (PI), in which the allele frequency is required to indicate whether the alleged father is the true biological father of the child. Validated 8TR loci have been analysed extensively in various populations for forensic application. In this research study, population genetic studies were carried out for three 8TR loci, namely D168539, D7S820 and D13S317 for Temiar population by using a multiplex PCR reaction followed by 4% polyacrylamide gel electrophoresis (PAGE) and silver staining. The heterozygosity (H) for these three loci, i.e. D16S539, D78820 and D138317 are 0.9568, 0.9132 and 0.7427 respectively. Power of Discrimination (PD) for these three loci are 0.9135 (D16S539), 0.9331 (D78820) and 0.9050 (D13S317); PowerofExclusion (PE) was found to be 0.5192 (0168539), 0.6034 (078820) and 0.4053 (D13S317) respectively. The Cumulative Discrimination Power (CDP) for the three 8TRs for Temiar population is 0.99903.