AlGaN-based UVC LEDs have now received numerous attentions due to their ability to eliminate coronaviruses which cause COVID-19 disease. It is therefore essential to improve the efficiency of the LEDs to make them compatible for large scale applications. One of the major challenges to improve the efficiency is to reduce the dislocation density in AlN layer; the base layer for the LEDs, to be below 109 cm-2 . Thus far, many works have been proposed to reduce the dislocation in the AlN layer. However, by properly adjusting the AlN nucleation time in the growth of the AlN layer, the dislocation can be reduced. The effect might be more significant with TMAl preflow assistance, which is applied after the growth of the nucleation. In this work, we will present the effect of the nucleation time with the assistance of TMAl preflow on reducing the dislocation density in the overgrown AlN layer. With 60 seconds of nucleation, the density of the dislocation in the AlN layer can be as low as 9.0 x 108 cm-2 . In addition. the role of the TMAl preflow assistance will be justified. The AlN layer was subsequently used to grow a 255 nm UVC LED. The diode characteristic and CL emission of the LED will be discussed towards the end of the presentation.