| ชื่อเรื่อง | : | Characterization Of A Highly Active Polyhydroxyalkanoate Synthase |
| นักวิจัย | : | Chuah, Jo-Ann |
| คำค้น | : | QH1 Natural history (General - Including nature conservation, geographical distribution) |
| หน่วยงาน | : | Universiti Sains Malaysia, Malaysia |
| ผู้ร่วมงาน | : | - |
| ปีพิมพ์ | : | 2555 |
| อ้างอิง | : | http://eprints.usm.my/44792/1/CHUAH%20JO-ANN.pdf , Chuah, Jo-Ann (2012) Characterization Of A Highly Active Polyhydroxyalkanoate Synthase. PhD thesis, Universiti Sains Malaysia. |
| ที่มา | : | - |
| ความเชี่ยวชาญ | : | - |
| ความสัมพันธ์ | : | http://eprints.usm.my/44792/ |
| ขอบเขตของเนื้อหา | : | - |
| บทคัดย่อ/คำอธิบาย | : | Polyhydroxyalkanoate (PHA) synthase from a locally isolated Chromobacterium sp. USM2 (PhaCCs) exhibited superior polymerizing ability and broad in vivo substrate specificity with preferences for short chain length (SCL) [3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV)] and medium chain length (MCL) [3-hydroxyhexanoate (3HHx)] monomers. For further characterization of the synthase, a Strep2-tagged PhaCCs for expression in and purification from Escherichia coli, was constructed in this study. In vitro enzymatic assay revealed an activity of 253 ± 13 U/mg for polymerization of 3-hydroxybutyryl-coenzyme A (3HB-CoA), which was approximately fivefold higher than that of model PHAproducing strain Cupriavidus necator (39 ± 5 U/mg). |
| บรรณานุกรม | : |
Chuah, Jo-Ann . (2555). Characterization Of A Highly Active Polyhydroxyalkanoate Synthase.
กรุงเทพมหานคร : Universiti Sains Malaysia, Malaysia. Chuah, Jo-Ann . 2555. "Characterization Of A Highly Active Polyhydroxyalkanoate Synthase".
กรุงเทพมหานคร : Universiti Sains Malaysia, Malaysia. Chuah, Jo-Ann . "Characterization Of A Highly Active Polyhydroxyalkanoate Synthase."
กรุงเทพมหานคร : Universiti Sains Malaysia, Malaysia, 2555. Print. Chuah, Jo-Ann . Characterization Of A Highly Active Polyhydroxyalkanoate Synthase. กรุงเทพมหานคร : Universiti Sains Malaysia, Malaysia; 2555.
|
